The Chytrid fungus (Batrachochytrium dendrobatis) is a recognized global threat worldwide affecting numerous species of amphibians. Frogs and toads of Pampa's grasslands are not an exception. Chytrid fungus has been documented in several species of toads and frogs in Pampa grasslands, including endemic threatened species as the Darwin’s blackish toad (Melanophryniscus nigricans), and the Argentine horned frog (Ceratoprhys ornata).

Several solutions have been proposed to control Chytrid fungus, including the use of chemical compounds, but few initiatives were tested in the field. We will concentrate our efforts in highland grassland remnants of the Sierra del Tigre Natural Reserve and neighbour areas.

In this project we will conduct a preliminary experience by testing the use of colloidal silver in experimental ponds. We are looking for an effective & eco-friendly treatment to control the Chytrid fungus. We will also manage the breeding ponds to reduce or eliminate the presence of chytrid fungus in the Reserve and neighbour areas. Finally, we will create a document to control chytrid fungus in the “Sierra del Tigre” Natural Reserve, and we will work within young generations regarding the importance of natural resource use to the benefit of amphibians and their habitats through talks with local schools.