This project is the continuation of our first Rufford grant on Preuss’s guenon which is a poorly studied diurnal primate, classified as Endangered on the IUCN Redlist and a class A species (totally protected) in the Cameroon National Wildlife Legislation. Interviews with hunters highlighted Preuss’s guenon vulnerability to anthropogenic pressures in the eastern part of Ebo forest which we did not survey. The Ebo forest area may be one of the species last strongholds in Cameroon, unfortunately is subjected to hunting pressures, exploitation concerns and road development recently. Our limited survey efforts yielded incomplete knowledge on the distribution, status and responses to anthropogenic factors in the Ebo forest. With no current legal conservation status, there is a continued risk that hunting pressure, logging and other land use changes would threaten the local biodiversity of the Ebo forest. In light of this context, it is of great significance to collect quantitative and qualitative data on the ecology, distribution and status of the Preuss’s guenon in the Ebo forest to provide vital information for biodiversity conservation.